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Slipknotted and unknotted monovalent cation-proton antiporters evolved from a common ancestor

Fig 4

Identified structural differences between the slipknotted and unknotted proteins.

(A) Schematic representation of the full length two-domain slipknotted protein. Two domains are connected by the long loop. The conserved core region is colored in green. TM helices are enumereted as in the structure (PDBID: 5a1s). (B) Schematic representation of the unknotted protein. Similarly, unknotted protein is composed of two domains. Conserved core is colored in green. TM helices are enumereted as in the structure (PDBID: 4bwz). (C) Structure of a single domain of the slipknotted protein (PDBID: 5a1s). (D) Superposition of the domains A of the slipknotted and unknotted proteins. (E) Structure of a single domain of the unknotted protein (PDBID: 4bwz). (F) Superposition of the fragments of domains A of the slipknotted and unknotted proteins.

Fig 4

doi: https://doi.org/10.1371/journal.pcbi.1009502.g004