Towards integration of time-resolved confocal microscopy of a 3D in vitro microfluidic platform with a hybrid multiscale model of tumor angiogenesis
Fig 9
Each column depicts the data (green), model prediction (red), and overlap of the two (yellow) on days 5, 7, and 9, respectively. Each row shows the predicted centerlines using three different thresholds: 99% prediction (Fig 9A-C: top row), 95% prediction (Fig 9D-F: middle row), and 90% prediction (Fig 9G-H: bottom row) of the simulations. While the data exhibits vessel anastomosis at Day 7, the model prediction only depicts anastomosis in the 1% prediction at Day 7 (Fig 9B) and Day 9 (Fig 9C and 9F and 9I). Fig 9J and 9K shows the prediction of the average centerline distance from model to data and from data to model, respectively. The average centerline distance is normalized by the length of the longest sprout in this region at day 3. This results in a normalized length of less than 10% from model to data and a normalized length of less than 20% from data to model. However, at day 9 the complexity in the vascular network, specifically the vascular remodeling that eliminates the anastomosis, is beyond the capabilities of the model to predict.