Towards integration of time-resolved confocal microscopy of a 3D in vitro microfluidic platform with a hybrid multiscale model of tumor angiogenesis
Fig 8
Local region 1: Calibration of stalk cell growth rate.
Fig 8A-C show the centerlines calculated from the model best fit (red), the data (green), and the overlap (yellow) on day 3 (used to inform the initial conditions), day 5, and day 7 (used to calibrate the stalk cell growth rate). The ABM is shown in Fig 8D-F with tip cells in green, stalk cells in cyan, endothelial cells in red, tumor cells releasing VEGF in orange, and tumor cells not releasing VEGF in blue. In Fig 8G, we show the calibrated PDF and the Gaussian fit of the stalk cell growth rate. The best fit model recapitulates the general structural features of the data without allowing additional sprouts to form. We also note that from day 7 (depicted in Fig 8F) to day 9, only the two tumor cells in the bottom right of the domain continue to release VEGF, guiding the sprout migration to the bottom right of the domain.