Towards integration of time-resolved confocal microscopy of a 3D in vitro microfluidic platform with a hybrid multiscale model of tumor angiogenesis
Fig 7
Vascular regions for local calibration.
Fig 7A shows a RBG binarized image of vascular structure of day 3 in the microfluidic platform. Fig 7B-E depict a specific local region segmented over time (blue box in Fig 7A) with vessels shown in red and tumor cells shown in green from days 3, 5, 7, and 9. Fig 7F-I show the corresponding centerline segmentation of Fig 7B-C, computed from a parallel thinning algorithm. The local analysis flowchart is shown by model initialization using the day 3 centerline, model calibration utilizing days 5 and 7, and model prediction of the centerline from day 9.