Time to revisit the endpoint dilution assay and to replace the TCID50 as a measure of a virus sample’s infection concentration

doi:10.1371/journal.pcbi.1009480

Time to revisit the endpoint dilution assay and to replace the TCID₅₀ as a measure of a virus sample’s infection concentration

Fig 5

midSIN’s estimate of a sample’s infection concentration based on a single dilution.

Simulated example of an ED plate with an inoculation volume of V_inoc = 0.1 mL. Instead of serial dilutions, a single dilution () is used, and either 1, 2 or 3 well(s) out of the 4 replicate wells are infected. As the fraction of infected wells increases, the uncertainty on the estimate (68% and 95% CIs) decreases, and the posterior distribution becomes more symmetric (Normal-like). Other features are as explained in the caption of Fig 1. The RM and SK methods cannot provide an estimate for these outcomes.

doi: https://doi.org/10.1371/journal.pcbi.1009480.g005