Time to revisit the endpoint dilution assay and to replace the TCID50 as a measure of a virus sample’s infection concentration
Fig 2
Quantification of RSV sampled from in vitro infections.
Each row corresponds to a different experiment (mock-yield [my] or single-cycle [sc]) and sampling time point (e.g., 8 h, 36 h), and each sample was measured in duplicate (rep1, rep2). These data were collected from in vitro infections with the RSV A Long strain, and were previously reported in [11]. The ED measurement experiment were conducted using a plate layout of 11 dilutions, with 8 replicates per dilution, an inoculum volume of Vinoc = 0.1 mL, serial dilutions from to
, separated by a dilution factor of 10−0.5.