Engineering gene overlaps to sustain genetic constructs in vivo
Fig 3
Large overlapping reading frames can be created in many genes.
(a) Potential Riboverlaps in E. coli: cumulative distribution of the first position at which an overlapping reading frames can be created, for all genes of E. coli MG1655. Different colors represent different levels of stringency: 0 (blue), 1 (green), or 2 (red) amino acid changes allowed. (b) Potential Riboverlaps in other bacterial species: relative size of the largest overlapping frame that can be created. All coding sequences of each organism are averaged, and intermediate level of stringency (1 AA change) was chosen. Error bars represent 95% confidence intervals for all coding sequences of the focal organism. (c) Potential Riboverlaps in all COG (Cluster Of Genes) categories. Pooling the 156,542 coding sequences of 50 bacterial species, average relative size of the largest overlapping frame can be created, for each COG category. Error bars represent 95% confidence intervals for all proteins attributed to the focal functional category.