Comprehensive analysis of lectin-glycan interactions reveals determinants of lectin specificity
Fig 4
Determinants of global lectin specificity are shared for similar glycans.
Similar glycans have similar patterns of enriched and depleted interaction features as observed by Pearson correlations between weighted WMW feature effect sizes. Panel A shows the correlogram from all 221 interaction feature effect sizes, clustered by Pearson correlation coefficient. Panels B-D show heatmaps of the interaction feature effect sizes with features in the columns and ligands in the rows clustered by Pearson correlation. Features that are statistically significant by the weighted WMW tests (q < 0.01) and in at least the 75th percentile of median feature-type-stratified importance from the random forest models are indicated with bullet points. The color bars present along the columns indicated the subcategory of the feature and the parameter threshold used when extracting the feature. The color bars along the rows indicate the identity of the terminal saccharide in the glycan and the number of saccharides present. Clusters discussed include sialic acid glycans (purple boxes), mannose and glucose (cyan boxes), lactose and N-acetyllactosamine (orange boxes), and fucose and terminal fucose containing glycans (red boxes). Interestingly, N-acetylglucosamine interactions are more similar to interactions with galactose while N-acetylgalactosamine interactions are more similar to interactions with glucose. The dark green boxes indicate distinct patterns in the 3D pockets of interactions with high mannose. Glycan symbols follow the SNFG system.