Sarc-Graph: Automated segmentation, tracking, and analysis of sarcomeres in hiPSC-derived cardiomyocytes
Fig 8
Example performance on experimental data E3 and E4: Raw image visualization; z-disc and sarcomere segmentation; Sarcomeres that are tracked for 1/3 or more of the movie, red corresponds to a contracted state, blue corresponds to a relaxed state (frame 50 for E3, frame 40 for E4); Sarcomeres that are tracked for 3/4 or more of the movie, red corresponds to a contracted state, blue corresponds to a relaxed state (frame 50 for E3, frame 40 for E4), note that these sarcomeres are included in the time series analysis; Individual time series data for normalized sarcomere length; Average deformation behavior; Illustration of the data represented as a spatial graph, color corresponds to sarcomere orientation; Normalized sarcomere time series cross-correlation score plotted as a function of the distance along the network. S3 and S4 Movies are included for further visualization.