Sarc-Graph: Automated segmentation, tracking, and analysis of sarcomeres in hiPSC-derived cardiomyocytes
Fig 4
Data analysis from segmentation and tracking: (a) Tracked elements are treated as fiducial markers and used to construct an average deformation gradient F (see Eqs 1–4); (b) z-discs and sarcomeres are tracked independently and used to construct a spatial graph (z-discs are “nodes” and sarcomeres are “edges”, edge color corresponds to sarcomere orientation, node color corresponds to correlation in sarcomere orientation) to measure correlations based on network distance; (c) Automated analysis of time series data is used to extract constants such as mean contraction time and number of peaks.