Sarc-Graph: Automated segmentation, tracking, and analysis of sarcomeres in hiPSC-derived cardiomyocytes
Fig 2
Creating synthetic data to test the image analysis code: (a) Define the geometry of z-discs (points) and sarcomeres (line segments) in three-dimensional (3D) space and prescribe the ground truth deformation for each sarcomere; (b) Approximate each z-disc as an oriented cylinder in 3D space; (c) Convert the 3D geometry into a voxel array with higher resolution in the x and y directions than the z direction; (d) Slice and project the voxel array to obtain a two-dimensional (2D) image. Image noise and blur can be added to the voxel array, 2D image, or both.