Ankyrin repeats in context with human population variation
Fig 6
(A) Normalised Shenkin divergence score per domain position (Eq 3) calculated from the MSA containing 7,404 sequences. Positions are coloured according to their normalised Shenkin score as the legend indicates; (B) Secondary structure assignment per position. Within each position, each coloured bar represents the frequency of the eight states defined by DSSP: α-helix, 310-helix, π-helix, β-bridge, β-strand, turn, bend and coil, observed for the residues with structural coverage at that column in the MSA. Most helices range from 5–11 and 15–23 and finish in 5-turns, usually at positions 12–13 and 23–24. Two β-turns are observed at positions 28–29 and 33–1; (C) Median residue relative surface accessibility per position, calculated from DSSP’s accessible surface area [42] as described in Tien et al [48]. Error bars indicate 95% CI of the median. Positions were classified according to the specified thresholds: surface (RSA ≥ 25%), partially exposed (5% < RSA < 25%) or buried (RSA ≤ 5%) [49].