Transcriptomic entropy benchmarks stem cell-derived cardiomyocyte maturation against endogenous tissue at single cell level
Fig 4
Entropy score enables cross-study and cross-species comparison of CM maturation status, and recapitulates gene trends in CM maturation.
A. Workflow for computing entropy score from high quality scRNA-seq datasets. B. Entropy score for mouse and human in vivo CMs taken from publicly available datasets. HEW = human embryonic week, HPY = human postnatal year. C. Pearson correlation between entropy score and calculated pseudotimes for our maturation reference dataset for three trajectory inference methods: Monocle 2, Slingshot, and SCORPIUS. D. Venn diagram showing overlap in identified differentially expressed genes between entropy score and trajectory inference methods. Differentially expressed genes were identified by fitting generalized additive models to gene trends over the corresponding pseudotime in Monocle 2, and selecting genes with adjusted p-value < 0.05. E. Gene expression trends over entropy score for genes involved in CM maturation, including sarcomeric, cell cycle, metabolism, and calcium handling genes.