Transcriptomic entropy benchmarks stem cell-derived cardiomyocyte maturation against endogenous tissue at single cell level
Fig 3
Standardized cell and study filtration enables meta-analysis of CM maturation with entropy.
A. Mitochondrial gene fractions in mouse in vivo datasets. Datasets with unusually high proportions are highlighted in red and were removed from subsequent analysis. B. Subsampling of count depth in Dueck et al. dataset (the highest depth dataset in our analysis). We subsampled to a depth where the median number of genes remained > 1000. We subsequently computed the accuracy as deviation from baseline entropy. C. Unusually low entropy cells due to high top 5 gene percentage (top) or low depth (bottom) in the Churko et al. dataset. D. SingleCellNet CM scores for mouse in vivo datasets by timepoint. Cells are labeled based on whether their highest classification was for “cardiac muscle”.