Label-free imaging and classification of live P. falciparum enables high performance parasitemia quantification without fixation or staining
Fig 5
Commercial microscope with 405 nm excitation.
Equipping a commercial widefield microscope with 405 nm excitation generates high-contrast label-free images of infected RBCs. a) Cross-sectional view of our fiber-coupled, collimated light source used for near-UV excitation through the microscope transillumination port. b) Confusion matrix from the single-wavelength validation dataset of the classifier trained with 405 nm images from the commercial microscope. c) Results from human collaboration, ie. human correction of labels for only the cells with the lowest classifier confidence. All four graphs depict performance metrics as a function of the total percentage of cells corrected. Upper left: Overall accuracy, Upper right: False-Positive Rate, Lower left: Precision, and Lower right: recall. The human plus machine classifier achieves perfect performance after correction of 20% of the dataset. d) Example cell images of all four categories, acquired at 405 nm. From left to right: Healthy, Rings, Trophozoites, and Schizonts.