Improving the understanding of cytoneme-mediated morphogen gradients by in silico modeling
Fig 7
FRAP experiments to study the temporal gradient formation.
(A) Representative image of FRAP experiments in abdominal histoblast nests in which the signal is eliminated after photobleaching over a specific ROI. (B) Hh (Hh:GFP BAC) gradient profile shortly before bleaching in black and Hh signal recovery over time coded in a hot colormap; each step corresponds to 45 seconds. (C) Ptc (EnhancerPtcRed) expression profile shortly before bleaching in black and ptc signal recovery over time coded in a hot colormap, each step is 45 seconds. D) In silico signal evolution predicted for abdominal histoblast nests. E) A graphical comparison every 3 minutes between in silico simulations and experimental data. F) Simulations for cytonemes contacting while growing with a different proportion of triangular/trapezoidal cytoneme dynamics (10% triangles in light blue, 50% triangles in red, 90% triangles in dark blue). Scale bars: 15μm.