Improving the understanding of cytoneme-mediated morphogen gradients by in silico modeling
Fig 3
Experimental cytoneme characterization in Drosophila tissues.
(A) Wing imaginal disc cytonemes protruding from A (top left) and from P (top right) compartment cells marked with Ihog-RFP. Bottom panels show 3D reconstructions of a confocal Z-stack taken at the basal side of the tissue showing cytonemes protruding and from A (bottom left) and P (bottom right) compartment cells. (B) A confocal Z-stack taken from the apical to basal side of the abdominal histoblast epithelium with the A compartment marked with life-actin-RFP (red) and the P compartment marked with CD8GFP (green). (C) In vivo temporal sequence of abdominal histoblast cytonemes taken at one-minute intervals. Top image sequences show both A and P compartment labelled cytonemes (A in red, P in green), middle image sequences show a single channel of A compartment cytonemes, and bottom image sequences show the single channel of P compartment cytonemes. (D) Statistical violin plots of cytoneme length distribution in the A (blue) and the P (green) compartments in wing disc (left) and abdominal histoblast nest (right). Scale bars: 15μm.