Rigid-body fitting to atomic force microscopy images for inferring probe shape and biomolecular structure
Fig 6
Exhaustive search rigid body fitting to an HS-AFM experimental image of FlhAC ring [36].
(A) The stage plane estimated from the background regions of experimental AFM image. (B) The AFM image after the correction of stage plane. (C) The reference image used in the rigid-body fitting. It is taken from the left middle rectangular region of the image in (B). (D) The pseudo-AFM image generated by the estimation result with the correlation coefficient. (E) The absolute value of differences between (C) and (D). (F) The resulting FlhAC nonamer ring structure model on top of the reference AFM image. (G-I) The result of fitting using cosine similarity (G), pixel-RMSD (H), and correlation coefficient (I). The colors and shapes of the marker represents the angle of the probe. The angle is 5 (dot), 10 (triangle), 15 (diamond), 20 (thin diamond), 25 (x), and 30 degrees (hexagon), respectively. Within 6 trials using varying probe angles, the same probe radius is used.