Machine learning reveals mesenchymal breast carcinoma cell adaptation in response to matrix stiffness
Fig 7
Comparison of individual and clustered cells cultured at 64 kPa.
(a) Example images of individual and clumped cells cultured at 64 kPa. Cells stained with DAPI (blue) and WGA-FITC (green) to visualise cell nuclei and cytoplasm, and for E-cadherin (magenta). (b) Key differences between Single and Clumped cells. (c) Cell morph composition of Clumped cell population. (d) Mean intensity and mean edge intensity of E-cadherin in Single and Clumped cells. Schematic illustrations of the measurements are provided on the bottom. (e) Fraction of the total cell fluorescent signal located along the edge of a cell. Single and Clumped cells at 64 kPa are plotted separately. (f) TNBC cells (purple) displayed dramatically different morphological and colonisation patterns in the parenchymal (left) and stromal (right) compartments of our 3D liver tissue model. For (b), (d) and (e), values are mean, error bars indicate standard deviation. Significance of the difference assessed by Welch’s t-test (‘***’: p < 0.001, ‘**’: p < 0.01, ‘*’: p < 0.05).