Machine learning reveals mesenchymal breast carcinoma cell adaptation in response to matrix stiffness
Fig 3
Distinct adaptations to substrate stiffness by TNBC cells.
(a) An increase in cell density compared to baseline (dashed line) after cells were cultured for 24 hours on substrates with different stiffness values, n = 4 replicates per stiffness value. (b) Analysis of the association between the top 10 parameters modulated by substrate stiffness. Most measurements are related and thus are highly correlated with each other. (c) Changes in the distributions of NCR, cell perimeter, area, smallest diameter, circularity, and distance to the nearest cell in response to substrate stiffness. Distributions are reported using box plots: a box shows the median value, first and third quartiles, whiskers indicate median +/-1.5 * IQR. Significance of the difference assessed by Welch’s t-test (‘***’: p < 0.001, ‘**’: p < 0.01, ‘*’: p < 0.05), n = number of cells, see Table A in S2 Text.