Neuron tracing and quantitative analyses of dendritic architecture reveal symmetrical three-way-junctions and phenotypes of git-1 in C. elegans
Fig 7
Behavioral characterization of git-1 mutants.
A-C. Characteristic tracks obtained by automated video analysis of six animals over three minutes, recorded at 7.5 frames per second (fps), for WT, git-1(tm1962) and git-1(ok1848) animals, respectively. For each genotype, the animals were recorded on a single plate, and the obtained tracks were separated for clarity. Red arrowheads indicate events where the track was lost (see Materials and Methods). D. Crawling wavelength automatically extracted and averaged over a three-minute, 7.5 fps recording. n = 31 WT; n = 15 git-1(tm1962); n = 33 git-1(ok1848). Student’s t test, ***p < 0.0005. Error bars represent the standard deviation. Values remain significant when normalized per worm length (results not shown). E. Crawling speed normalized by total worm length, automatically extracted and averaged over a three-minute, 7.5 fps recording. n = 31 WT; n = 15 git-1(tm1962); n = 33 git1(ok1848). Student’s t test, **p < 0.005. Error bars represent the standard deviation. Values remain significant when normalized per worm length (results not shown). F. Percentage of worms executing a harsh-touch-induced escape response. Results are not significant (p > 0.01) compared to WT for all genotypes except the harsh-touch insensitive mec-3(e1338). As a negative control, the gentle-touch insensitive mec-4(e1611) animals are used. n = 310 WT; n = 312 git-1(tm1962); n = 250 git-1(ok1848); n = 150 mec-4(e1611); n = 70 mec-3(e1338), n = 4 independent experiments for WT and git-1(tm1962) mutants, n = 3 independent experiments for git-1(ok1848) mutants and n = 2 independent experiments for mec-4 and mec-3 mutants. Fisher’s exact test, ***p < 0.0001. Error bars represent the standard deviation.