Neuron tracing and quantitative analyses of dendritic architecture reveal symmetrical three-way-junctions and phenotypes of git-1 in C. elegans
Fig 4
Morphological characterization of the wild-type PVD.
A. Distribution of PVD elements by the azimuthal angle, ϕ, and midline orientation angle θ. Four distinct morphological classes are found: Class 1 (red), class 2 (green), class 3 (blue) and class 4 (yellow) (n = 10). B. The percentage of each of the morphological class found in (A); (n = 10). C. Visual example of the algorithmically-derived classification (same neuron as Fig 1A), closely resembling conventional branch-order classification scheme (see Fig 1C). D. Distribution of neuron length elements along the midline, classified as in C and averaged across worms (n = 10). E. Mean curvature of neuronal elements for each morphological class. Class 4 elements are the most curved, whereas class 3 elements are more curved than class 1 and 2, but less than class 4. The difference between class 2 and 4 is also statistically significant. Statistics were calculated using the nonparametric Mann–Whitney test. ***p < 0.0005. Error bars show the standard deviation (n = 10). Same neuron as Fig 1A. F. Example of a PVD neuron color-coded for curvature. Same neuron as Fig 1A.