A gap-filling algorithm for prediction of metabolic interactions in microbial communities
Fig 4
Graphical representation of the community of Dehalobacter sp. CF and Bacteroidales sp. CF50 after the application of the community gap-filling method.
The best solution calculated by the community gap-filling algorithm predicted that the metabolic model of Dehalobacter sp. CF consumes chloroform (CF), while the metabolic model of Bacteroidales sp. CF50 consumes lactate (LAC) from the common medium. Dehalobacter sp. CF consumes H2 and CO2 produced by Bacteroidales sp. CF50, and uses part of the consumed H2 in order to respire chloroform (CF) to chlorine (Cl) and dichloromethane (DCM). The two models exchange acetate (AC), malate (MAL) and pyruvate (PYR) as expected from experimental studies. The algorithm also predicted the exchange of specific amino acids (3 letter code) between the models. The non-dashed arrows represent intracellular metabolite flow, while the dashed arrows represent exchange reactions. The thickness of the dashed arrows represents the relative order of magnitude of the calculated fluxes for the exchange reactions.