Benchmarking of tools for axon length measurement in individually-labeled projection neurons
Fig 8
Error probability distribution for axon length estimation by planes-based stereology.
A. Probability of ±5% (first row) and ±10% (second row) axon length estimation error for different combinations of distance and step, and for axon classes 1 to 4 (columns); probability is marked by color gradient, from 0.25 to 0.92 (see color bar). Probabilities for classes 1, 2, and 3 are plotted with the same colored-scale. White dots point to the cases for distance = 18 μ and step = 110 μ. Values were obtained through linear interpolation (Adjusted R-squared for 5% error: class 1 = 0.80, class 2 = 0.86, class 3 = 0.81, class 4 = 0.91; Adjusted R-squared for 10% error: class 1 = 0.83, class 2 = 0.83, class 3 = 0.85, class 4 = 0.93). B. Error probability distribution for the particular cases detailed in A. The areas below the curves are the probabilities shown in A and pointed with the white dots.