Extracting temporal relationships between weakly coupled peptidergic and motoneuronal signaling: Application to Drosophila ecdysis behavior
Fig 1
CCAP neuron and motoneuron activity.
(a) Single-plane calcium imaging of GCaMP3.2-expressing CCAP neurons. (b) Projection of 5 images from different planes, of GCaMP3.2-expressing CCAP neurons and motoneurons. The same scale bar from panel (a) applies. (c) Time series of signals from calcium sensor in AN1-AN4 α CCAP neurons and motoneurons recorded in a single experiment; time zero corresponds to moment of ETH-stimulation. The letter indicates the side (left [L] and right [R]), while the number indicates the abdominal segment of the neurons. Vertical marks denote the onset of oscillatory activity, as detected by the procedure indicated in Methods. (d) Mean time of onset of α CCAP neuron and motoneuron activity, for each of 9 separate experiments, showing temporally close values between populations. “MN” and “CCAP” indicate motoneurons and α CCAP neurons, respectively. Each MN-CCAP pair corresponds to a single experiment, with its 2 MN and 8 CCAP recordings. Box plots indicate intra-experiment median and quartiles.