Sequence deeper without sequencing more: Bayesian resolution of ambiguously mapped reads
Fig 1
Summary of the SmartMap analysis workflow and algorithm.
(A) Flowchart outlining the workflow for traditional ChIP-seq (or ICeChIP-seq) analysis [29–31] utilizing only unireads (left, green) vs. the workflow for SmartMap analysis utilizing multireads with an iterative Bayesian reweighting algorithm (right, blue). (B) Schematic showing the Bayesian reweighting algorithm utilized in the SmartMap analysis. Each mapping associated with a read is assigned a weight such that the weight is greater for those mappings associated with loci of greater map weight density. For more detailed description of the algorithm, see Methods.