A novel yeast hybrid modeling framework integrating Boolean and enzyme-constrained networks enables exploration of the interplay between signaling and metabolism
Fig 1
Schematic representation of the hybrid model.
The hybrid model consists of a vector-based Boolean module of nutrient signaling and an enzyme constrained module of the central carbon metabolism. The Boolean module is a dynamic module including Snf1, PKA, and TORC1 pathway as well as crosstalk between them. The dynamic module reaches a steady-state and the activity of the transcription factors acts as input in a regulatory network constraining the enzyme constraint model of the central carbon metabolism. The solution is used to determine the activity of the Boolean input.