Next-generation yeast-two-hybrid analysis with Y2H-SCORES identifies novel interactors of the MLA immune receptor
Fig 6
Experimental validation of the interaction between the MLA6 NLR receptor and barley targets.
A) Binary Y2H test between MLA6 and fourteen barley prey targets showing the diploid controls (SC-LW), stringent interaction (SC-LWH + 1 mM 3AT), and tests with empty-bait vector to show the specificity of the interaction. Preys are ranked in order of Y2H SCORES Borda ensemble. Rows followed by an asterisk (*) designate protein was also identified with MLA61-161 as shown in S4 Fig. Rows with two HORVU I.D.s indicate duplicate copies in the genome. B) Y2H-SCORES performance and PCA of scores calculated from the MLA6 datasets. C) Prediction of interologs for the validated Y2H-SCORES interactions with MLA6. Significant trans-eQTL associations (q-value<0.001) [39] with the Mla1 (locus coordinate 1H.05) and MlLa (locus coordinate 2H.67) are color coded. Biological processes associated with interologs are depicted in boxes next to each group.