Next-generation yeast-two-hybrid analysis with Y2H-SCORES identifies novel interactors of the MLA immune receptor
Fig 3
Principle of Y2H-SCORES and performance in an ideal scenario.
A) Y2H-SCORES is comprised of the enrichment score, which detects changes in prey proportions in selected and non-selected conditions; the specificity score, which measures differences in the prey enrichments with different baits under selection; and the in-frame score, which identifies the enrichment of prey reading frames under selection, assigning higher values to in-frame preys. B) to D) ROC curves of the enrichment, specificity, and in-frame scores in an ideal scenario. Colored sections represent 95% confidence intervals for the score values 0.7, 0.5, and 0.3. E) PCA of the Y2H-SCORES calculated under the ideal scenario.