Next-generation yeast-two-hybrid analysis with Y2H-SCORES identifies novel interactors of the MLA immune receptor
Fig 2
Effect of count normalization in Y2H-NGIS.
A) PCA analysis of raw read counts and B) TPM, C) RUVs, D) Median-of-ratios and E) Library size normalized reads for selected (left) and non-selected (right) samples for five bait screenings (color coded). F) Boxplot of the pairwise correlation coefficients for raw and normalized read counts for all samples in non-selected and, separately, selected conditions. G) Coefficient of variation (CV) for each prey using different normalization methods in the three MLA6 baits and two luciferase screenings. Higher CV values may indicate poor performance because of a high variation between replicates.