MAUI (MBI Analysis User Interface)—An image processing pipeline for Multiplexed Mass Based Imaging
Fig 2
Processing MIBI data using MAUI results in signal similar to Immunohistochemistry.
Shown are serial sections from human colon stained for CD8 by IHC (top left) or MIBI (top right). White arrows indicate cytotoxic T cells in the lamina propria of the gut. Red arrows indicate imaging artifacts including background and cross-channel contamination (bottom left). After processing by MAUI (bottom right) the CD8 signal mirrors the expected histological staining pattern by IHC. Scalebar equals 40μm.