MAUI (MBI Analysis User Interface)—An image processing pipeline for Multiplexed Mass Based Imaging
Fig 1
Workflow of Multiplexed Ion Beam Imaging User Analysis Interface (MAUI).
Tissue specimens are stained using a mixture of antibodies labeled with elemental mass tags. The samples are rasterized by a primary ion beam that releases the metals of the bound antibodies as secondary ions, which are recorded by a Time of Flight Mass Spec. This results in an n-dimensional image depicting protein expression in the field. Image processing includes removal of cross-channel contamination, noise and aggregates.