Calcium-vesicles perform active diffusion in the sea urchin embryo during larval biomineralization
Fig 8
Active myosinII signal is enriched in the ectodermal cells compared to the skeletogenec cells.
(A-F) Representative images showing active myosinII (MyoIIP) in normal embryos and VEGFR inhibited embryos. (A, D) active MyosinII in normal and VEGFT inhibited embryos, respectively. Arrowhead indicates the apical side of the ectodermal cells. (B, E) skeletogenic cells marker (6a9) in normal and VEGFR inhibited embryos, respectively. (C, F) overlay of the two markers, with indicated sections enlarged on the right. (G-H) quantification of the active myosinII signal. The number of red pixels (active myosinII) per marked area was measured (G). The active myosinII signal is significantly higher in the ectodermal cells compared to the skeletogenic cells and is unaffected by VEGFR inhibition (Dunn-Sidak test, p<0.0001, exact p-values are given in S1 Dataset). Based on 3 biological replicates where overall n = 27 normal embryos and n = 30 VEGFR inhibited embryos were studied.