Calcium-vesicles perform active diffusion in the sea urchin embryo during larval biomineralization
Fig 7
Actin filaments are detected around the spicule and are enriched in the ectodermal cells compared to the skeletogenec cells.
(A-F) Representative images showing actin filaments in normal embryos (A-C) and VEGFR inhibited embryos (D-F). Phalloidin was used to stain f-actin (A and D) and 6a9 was used to mark the skeletogenic cells (B and E). Arrow in A marks the spicule, arrowheads in A and D mark the apical side of the ectodermal cells. In C and F we present the overlay of the phalloidin and the skeletogenic marker, with indicated sections enlarged on the right. (G-H) quantification of the phalloidion signal. The number of red pixels (phallodin signal) per marked area was measured (G). The phallodin signal is significantly higher in the ectodermal cells compared to the skeletogenic cells and it unaffected by VEGFR inhibition (Dunn-Sidak test, p<0.0001, exact p-values are given in S1 Dataset). Based on 3 biological replicates where overall n = 24 normal embryos and n = 30 VEGFR inhibited embryos were studied.