Calcium-vesicles perform active diffusion in the sea urchin embryo during larval biomineralization
Fig 4
Vesicle volume is larger in the skeletogenic cells compared to the ectodermal cells and is significantly larger under VEGFR inhibition.
(A-C) An example for the image processing involved in the quantification of vesicle volume in the ectodermal vs. skeletogenic embryonic domains. (A) Raw image rendering of calcium vesicle detection. (B) Demonstration of the automated vesicle detection (segmentation) overlaid on the image in (A). (C) Manual identification of ectodermal region in red, rendered along with raw image frame. (D) Comparison of vesicle sizes in ectodermal and skeletogenic cells in control and VEGFR inhibition. The total number of vesicle measured in the control skeletogenic cells: 815, ectodermal cells: 3719; VEGFR inhibition skeletogenic cells: 1530, ectodermal cells: 8621. Each box plot shows the average (white square), median (middle line), the first and the third quartiles (the 25th and 75th percentiles, edges of boxes) and outliers (black dots). Vesicle volume is significantly higher in the skeletogenic cells compared to the ectodermal cells and VEGFR inhibition significantly increases vesicle volume in both cell types. (Dunn-Sidak test, p<0.0001, exact p-values are given in S1 Dataset).