Extracellular matrix density regulates the formation of tumour spheroids through cell migration
Fig 1
Schematic representation of the experimental configurations.
Using the same experimental conditions, consisting of a microfluidic device in which cells were seeded in a collagen matrix, it was possible to characterize cell motility and cluster formation as two distinct processes. The results recorded at day 1 provided the data used to define locomotive forces and, consequently, cell-matrix interactions. Furthermore, the results from the 5th day of experiments were used to calibrate cell-cell interactions.