Network potential identifies therapeutic miRNA cocktails in Ewing sarcoma
Fig 6
We identified miR-483–3p, miR-5695, and miR-4514s as the optimal 3-miRNA cocktail for Ewing Sarcoma therapy.
We identified cocktails that are predicted to maximally downregulate target genes (red shading on the figure), while avoiding downregulation of essential housekeeping genes to limit toxicity (blue shading on the figure). Panel A. shows the targeting heatmap for the best predicted cocktail for cell line A673. The miRNA that make up the cocktail are presented on the y-axis. Putative gene targets are highlighted on the x-axis. Lines that span multiple miRNAs occur when a gene is downregulated by 2 or more miRNAs in the cocktail. Panel B. shows a histogram of the number of microRNA that target a given housekeeping gene in the best cocktail. Panel C. displays the targeting heatmap for the worst-performing cocktail for cell line A673 among those tested for reference. Panel D. shows a histogram of the number of microRNA that target a given housekeeping gene in the worst predicted cocktail. Panel E shows a bar graph showing the miRNA that most frequently appear in either the bottom or top 10 predicted cocktails (averaged across cell lines) for Ewing Sarcoma therapy.