Network potential identifies therapeutic miRNA cocktails in Ewing sarcoma
Fig 2
Simplified schematic of our computational pipeline.
We defined a measure of tumor state, which we term network potential (Eq 1), based on both mRNA gene expression and the underlying protein-protein interaction (PPI) network. Next, we ranked mRNA targets based on their contribution to network potential of each cell line, aiming to approximate the relative importance of each mRNA to network stability. After identifying these mRNA targets, we then identified miRNA and miRNA cocktails that preferentially acted to repress the most influential of the ranked mRNA targets, with the aim of defining synthetic miRNA-based therapy for down-regulation of these targets.