Wide range of metabolic adaptations to the acquisition of the Calvin cycle revealed by comparison of microbial genomes
Fig 4
Tracing the evolution of Calvin cycle genome integration.
The panels show likelihood of ancestral Calvin cycle presence (node fill color; cyan indicates CBB-positive and brown indicates CBB-negative) in bacterial subtrees (A), positive correlation (Spearman r ≈ 0.45) to ancestral gene copy numbers (line color) of fructose-1,6-bisphosphatase (Fbp; EC 3.1.3.11) in Archaea (B), and strong negative correlation (Spearman r ≈ -0.85) to ancestral gene copy numbers (line color) of transcriptional regulator AraC (PF06719) in bacterial subtree 1 (C). Each leaf node (triangles) is one contemporary genome. Outer rings indicate genome taxonomic association. Scale bars show substitutions per site. Asterisks (*) indicate archaeal genomes encoding Rubisco activase CbbQ (PF08406). CbbQ is negatively correlated to the Calvin cycle in Archaea (r ≈ -0.62), which is explained by the fact that most of the archaeal genomes with CbbQ are CBB-negative (brown).