Biological impact of mutually exclusive exon switching
Fig 4
Structural and functional analysis of the MXE splicing dataset.
(A) The surface exposure of the MXE events compared to random expectation. The first quartile, median, and the third quartile of the population were indicated with lines. NACCESS was used to calculate the relative accessible surface (rASA) of amino acids. Amino acid residues were considered to be exposed if the rASA value was above 10%. (B) The surface exposure of the MXE variable residues compared to the MXE non-variable residues. We compared the solvent exposure of the variable residues versus the solvent exposure of the MXE conserved residues for each MXE event. NACCESS was used to calculate the relative accessible surface (rASA) of amino acids. Amino acid residues were considered to be exposed if the rASA value was above 10%. (C) Distribution of McLachlan scores for the variable residues. For each MXE, we summed up all the McLachlan similarity scores for a set of variable residues and divided by the total number of mapped variable residues to normalise the score. (D) Proximity of MXE variable residue clusters to amino acids assigned to different functional classes (indicated by Icons) CSA = catalytic residues from the Catalytic Site Atlas, PPI = protein interaction sites and PSI = protein-small molecule interactions). A breakdown of this Fig by individual species is available in S10 Fig. We used the Z-score test to compute the statistical significance. Where appropriate the level of statistical significance is shown above each type of functional site.