A kinetic model of the central carbon metabolism for acrylic acid production in Escherichia coli
Fig 9
Comparison between the original acrylic acid (AA) production with the results obtained for the mutants developed with the optimisation strategies identified.
(A) AA production using glycerol pathway. Mutant 0 represents the model with the heterologous pathway, and Mutant 1 the same model with a 45-fold increase in the Vmax of the glycerol-3-phosphate dehydrogenase (G3pD) reaction; (B) AA production using malonyl-CoA pathway. Mutant 0 represents the model with the heterologous pathway, and Mutant 1 the same model with a 2.5-fold increase in the Vmax of the acetyl-CoA carboxylase (AccC); (C) AA production for the β-alanine pathway. Mutant 0 represents the model with the heterologous pathway, and Mutant 1 the same model with a 50-fold increase in the Vmax of the aspartate aminotransferase (AspAT).