A kinetic model of the central carbon metabolism for acrylic acid production in Escherichia coli
Fig 8
Flux Control Coefficients (FCC) results for acrylic acid formation, where the reaction with the most impact in the yield is highlighted in red.
(A) Results for the glycerol pathway. According to the coefficients, the reaction with the most impact is the glycerol-3-phosphate dehydrogenase (G3pD), which due to its positive FCC is a potential target for overexpression; (B) Results for the malonyl-CoA pathway. The results showed that the acetyl-CoA carboxylase (AccC) is a potential bottleneck in the pathway due to the positive FCC; hence, another target for overexpression; (C) Results for the β-alanine pathway. The highest FCC was for the aspartate aminotransferase (AspAT) which appears to be an ideal target for an overexpression.