Inferring the structures of signaling motifs from paired dynamic traces of single cells
Fig 5
Motif inference for the yeast stress response pathway under different environmental perturbations.
(A) Schematic of the system showing the fluorescent reporter system used. See ref. 21 for experimental details. Experimental data were used with author permission. (B) Oxidative Stress. 58 cells were treated with 0.1 mM H2O2. Far left, heatmap of the individual cell’s Msn2 activation, and a plot of the mean and standard deviation of the cells. Left middle, STRE response as reported by the CFP reporter. Right middle, heatmap and ball and stick representations of the significant sub-motifs. Single asterisks represent 50% confidence and double asterisks represent 80% confidence. Displayed as ball and stick representations are the 80% confidence connections. Far right, Simulated STRE response of the top preforming sub-motif. (C) Nutrient Stress. 60 cells were treated with a 0.1% glucose media. Far left, heatmap of the individual cell’s Msn2 activation, and a plot of the mean and standard deviation of the cells. Left middle, STRE response as reported by the CFP reporter. Right middle, heatmap and ball and stick representations of the significant sub-motifs. Single asterisks represent 50% confidence and double asterisks represent 80% confidence. Displayed as ball and stick representations are the 80% confidence connections. Far right, Simulated STRE response of the top preforming sub-motif. (D) Osmotic Stress. 28 cells were treated with 0.5 M KCl. Far left, heatmap of the individual cell’s Msn2 activation, and a plot of the mean and standard deviation of the cells. Left middle, STRE response as reported by the CFP reporter. Right middle, heatmap and ball and stick representations of the significant sub-motifs. Single asterisks represent 50% confidence and double asterisks represent 80% confidence. Displayed as ball and stick representations are the 80% confidence connections. Far right, Simulated STRE response of the top preforming sub-motif.