Rapid 3D phenotypic analysis of neurons and organoids using data-driven cell segmentation-free machine learning
Fig 4
Concentration response and aggregate multivariate phenotypic response of neurons treated with BH3-mimetics.
A) 2-dimensional PCA representation of the image feature space for neurons treated with BH3 mimetics (arrows, concentration increase) showing averaged treatment centroids colored by drug and concentration. B) Heatmap of the proportion of images of neurons treated with the indicated drugs assigned to individual clusters. ActD–Actinomycin D, STS–Staurosporine; for A: the colors correspond to the different treatment groups as indicated in the legend for concentrations in nM.