miRNA normalization enables joint analysis of several datasets to increase sensitivity and to reveal novel miRNAs differentially expressed in breast cancer
Fig 10
Volcano plot of per-dataset Differential Expression of ER positive vs ER negative from Fig 1.
Here we include a both joint normalized and per dataset results. We observe an overall increase in statistical significance as dark points tend to be higher on the y-axis than their corresponding-colored points (indicated by dashed lines), as would be expected from the increase in statistical power. In some miRNA this can come at the cost of a lower detected fold-change as compared to some individual datasets.