miRNA normalization enables joint analysis of several datasets to increase sensitivity and to reveal novel miRNAs differentially expressed in breast cancer
Fig 6
Functional experiment results.
Breast cancer cell lines were transfected with miRNA mimics (20nM) and assayed for functional effects 72 hours after transfection. A) Cell viability measured in MCF7 breast cancer cells. B) Apoptosis measured by levels of cleaved PARP (cPARP), HER2 and phosphorylated ERK (pERK) protein levels measured in KPL4 cells. The dashed lines indicate cut-off points that were considered significant (see Materials and Methods). Asterisks denote significant effects. Original data from b) are taken from [32].