miRNA normalization enables joint analysis of several datasets to increase sensitivity and to reveal novel miRNAs differentially expressed in breast cancer
Fig 3
Differential miRNA expression between ER positive and negative.
A CDF plot showing many more substantially differentially expressed miRNAs after normalization (red line) than before normalization (blue line), and substantially more than would be expected at random (compared to 20 random permutations of labels, dashed black lines). Also shown are dashed colored lines corresponding to each appropriate single-dataset Q values exemplifying the advantage of a joint-dataset analysis. Note that at Q = 10−18 we can find 10 miRNAs under AQuN but none under other normalization approaches or per dataset analyses.