miRNA normalization enables joint analysis of several datasets to increase sensitivity and to reveal novel miRNAs differentially expressed in breast cancer
Fig 2
Differential miRNA expression between ER positive and negative.
Volcano plot showing the fold change and corresponding Wilcoxon Rank-sum FDR corrected Q value ratio between the normalized and unnormalized datasets. Dashed arrow connects the unnormalized (gray circles) and normalized (red circles) results on a particular miRNA. High absolute values in X axis correspond to substantial difference in median expression between ER negative over ER positive samples (for a particular miRNA). High values in Y axis correspond to miRNAs that present substantial difference *after* normalization but not before. Low values in Y axis correspond to miRNAs that present substantial difference *before* normalization but not after. Vertical dashed lines represent a Fold change threshold of 2x (log2(2) = 1) and horizontal dashed lines represent a Q-value threshold of 0.05 (-log10(0.05)≅1.3).