A novel stochastic simulation approach enables exploration of mechanisms for regulating polarity site movement
Fig 9
3D particle-based simulations recapitulate 2D spatial Gillespie simulations results from Fig 8.
(A) Snapshots of 3D particle-based simulations for different values of k4a. Cdc42T molecules are shown as red dots on a spherical surface representing the cell membrane. Rate constants are estimated from the ones used in Fig 8B as described in the Methods. Parameters are presented in Table 1. (B) Patch centroid diffusivity as a function of k4a for 3D particle-based simulations (red) with GEF = 100. For comparison we also show results from 2D spatial Gillespie simulations in Fig 8. Error bars are standard errors from the least-squared fit used to compute Dpatch.