PIEZO1 and the mechanism of the long circulatory longevity of human red blood cells
Fig 9
Effects of high and low cell volume fractions (CVF) during capillary transits on the lifespan patterns of changes in cell volume and [Ca2+]i.
Reference pattern (black) modelled with CVF of 0.9 during capillary transits. Test curves (red and green) modelled with CVF of 0.00001 throughout transit-intertransit periods. PMCA decay rates (kCaP), as indicated in the figure (in min-1). Note how the reduction in kCaP from 8e-6 (red) to 6e-6 (green) prevents hyperdense collapse at CVF 0.00001 and restores pattern towards Reference curve (black). Inset: [Ca2+]i values (CCa2+) recorded at the end of PIEZO1 open states on the last model iteration of each day (col 19 of the transit csv files). Note the increasing divergence between REF (black) and low-CVF (red) curves leading to hyperdense collapse. Late reversal of [Ca2+]i results from terminal rehydration of the cells.